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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and therefore may add to tumorigenesis. This protein was observed to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in selling cell-cycle development inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two added splice variants were described but in depth nucleotide sequence information will not be however out there.
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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified by the antibody.
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This information outlines even more doable optimization of mobile and immunoassay parameters to be certain the best possible results are...
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This tutorial outlines even further achievable optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Focus on Facts Cyclin E belongs on the hugely conserved cyclin loved ones, whose associates are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells development by means of S phase.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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